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Product Name
ETV6 Polyclonal Antibody
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Description
Polyclonal antibody to ETV6
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Tested applications
WB, IHC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
ETV6 antibody; TEL antibody; TEL/ABL antibody; THC5 antibody; ETS variant 6 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ETV6 (NP_001978.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100 -
Validations
Western blot - ETV6 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ETV6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunoprecipitation - ETV6 Polyclonal Antibody
Immunoprecipitation analysis of 200ug extracts of SW620 cells using 3ug ETV6 antibody . Western blot was performed from the immunoprecipitate using ETV6 antibody at a dilition of 1:500.
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Background
Transcriptional repressor; binds to the DNA sequence 5'-CCGGAAGT-3'. Plays a role in hematopoiesis and malignant transformation.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"