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Product Name
ETFA Polyclonal Antibody
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Description
Polyclonal antibody to ETFA
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ETFA antibody; EMA antibody; GA2 antibody; MADD antibody; electron transfer flavoprotein alpha subunit antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-333 of human ETFA (NP_000117.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100 -
Validations
Western blot - ETFA Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ETFA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - ETFA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate using ETFA antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ETFA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using ETFA antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ETFA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using ETFA antibody at dilution of 1:100 (40x lens).
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Background
Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"