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ETFA antibody

Cat.#: 110483

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Product Information

  • Product Name
    ETFA antibody
  • Documents
  • Description
    ETFA Rabbit Polyclonal antibody. Positive WB detected in mouse small intestine tissue, human ileum tissue, Y79 cells. Positive IF detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue. Positive FC detected in MCF-7 cells. Observed molecular weight by Western-blot: 30 kDa
  • Tested applications
    ELISA, WB, IHC, IF, FC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Alpha ETF antibody; EMA antibody; ETFA antibody; GA2 antibody; MADD antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ETFA recombinant protein (Accession Number: NM_000126). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with Catalog No:110483(ETFA antibody) at dilution of 1:1000

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with Catalog No:110483(ETFA antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:110483(ETFA antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:110483(ETFA antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of MCF-7 cells, using PNRC2 antibody Catalog No:110483 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of MCF-7 cells, using PNRC2 antibody Catalog No:110483 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    1X10^6 MCF-7 cells were stained with 0.2ug ETFA antibody (Catalog No:110483, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

    1X10^6 MCF-7 cells were stained with 0.2ug ETFA antibody (Catalog No:110483, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

  • Background
    Electron transfer flavoprotein (ETF) exists in the mitochondrial matrix as a heterodimer of 30 kD alpha subunits (ETFA) and 28 kD beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer. ETFA serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase.
  • References
    • Singaravelu R, Blais DR, McKay CS, Pezacki JP. Activity-based protein profiling of the hepatitis C virus replication in Huh-7 hepatoma cells using a non-directed active site probe. Proteome science. 8:5. 2010.
    • Muto T, Taniguchi H, Kushima R. Global expression study in colorectal cancer on proteins with alkaline isoelectric point by two-dimensional difference gel electrophoresis. Journal of proteomics. 74(6):858-73. 2011.
    • Han B, Stockwin LH, Hancock C, Yu SX, Hollingshead MG, Newton DL. Proteomic analysis of nuclei isolated from cancer cell lines treated with indenoisoquinoline NSC 724998, a novel topoisomerase I inhibitor. Journal of proteome research. 9(8):4016-27. 2010.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"