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Product Name
ETF1 Polyclonal Antibody
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Description
Polyclonal antibody to ETF1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ETF1 antibody; D5S1995 antibody; ERF antibody; ERF1 antibody; RF1 antibody; SUP45L1 antibody; TB3-1 antibody; eukaryotic translation termination factor 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 278-437 of human ETF1 (NP_004721.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
IF1:50 - 1:200 -
Validations
Western blot - ETF1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ETF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.
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Background
Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"