-
Product Name
ETF1 antibody
- Documents
-
Description
ETF1 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, mouse kidney tissue, mouse testis tissue. Positive IF detected in Hela cells. Positive IHC detected in human pancreas cancer tissue, human testis tissue. Observed molecular weight by Western-blot: 45-48kd
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human, Mouse; other species not tested.
-
Alternative names
D5S1995 antibody; ERF antibody; ERF1 antibody; ETF1 antibody; Eukaryotic release factor 1 antibody; Protein Cl1 antibody; RF1 antibody; SUP45L1 antibody; TB3 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of ETF1 recombinant protein (Accession Number: XM_005271921). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:110482(ETF1 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:110482(ETF1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using ETF1 antibody Catalog No:110482 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
-
Background
Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome, which is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In eukaryotes a single class-1 translation termination factor eRF1 decodes the three stop codons: UAA, UAG and UGA. [PMID:7990965,20860996]
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"