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Product Name
ERp72 antibody
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Description
ERp72 Rabbit Polyclonal antibody. Positive IF detected in PC-3 cells. Positive IHC detected in human lung cancer tissue, human prostate cancer tissue. Positive WB detected in HepG2 cells, HeLa cells, L02 cells, mouse brain tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 72 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ER protein 70 antibody; ER protein 72 antibody; ERp 72 antibody; ERP70 antibody; ERP72 antibody; PDIA4 antibody; Protein disulfide isomerase A4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ERp72 recombinant protein (Accession Number: NM_004911). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110375(PDIA4 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:110375(PDIA4 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:110375(PDIA4 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of PC-3 cells, using PDIA4 antibody Catalog No:110375 at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green).
IP Result of anti-ERp72 (IP:Catalog No:110375, 4ug; Detection:Catalog No:110375 1:500) with HeLa cells lysate 2800ug.
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Background
PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.
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References
- Ye H, Zhao T, Tan YL, Liu J, Pallen CJ, Xiao ZC. Receptor-like protein-tyrosine phosphatase α enhances cell surface expression of neural adhesion molecule NB-3. The Journal of biological chemistry. 286(29):26071-80. 2011.
- Sun QC, Liu MB, Shen HJ. Inhibition by imatinib of expression of O-glycan-related glycosyltransferases and tumor-associated carbohydrate antigens in the K562 human leukemia cell line. Asian Pacific journal of cancer prevention : APJCP. 14(4):2447-51. 2013.
- Zhang X, Zuo X, Yang B. MicroRNA directly enhances mitochondrial translation during muscle differentiation. Cell. 158(3):607-19. 2014.
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