-
Product Name
ERK1/2 antibody
- Documents
-
Description
ERK1/2 Rabbit Polyclonal antibody. Positive IHC detected in human cervical cancer tissue. Positive IF detected in HepG2 cells, HeLa cells. Positive WB detected in HeLa cells, A431 cells, HEK-293 cells, HepG2 cells, HepG2/A431 cells, mouse brain tissue, mouse skin tissue, NIH/3T3 cells, rat skeletal muscle tissue, rat skin tissue. Observed molecular weight by Western-blot: 38-44 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human, Mouse, Rat; other species not tested.
-
Alternative names
ERK antibody; ERK 2 antibody; ERK1/2 antibody; ERK2 antibody; ERT1 antibody; MAP kinase 1 antibody; MAP kinase 2 antibody; MAP kinase isoform p42 antibody; MAPK 1 antibody; MAPK 2 antibody; MAPK1 antibody; MAPK2 antibody; p40 antibody; p41 antibody; p41mapk antibody; p42 MAPK antibody; P42MAPK antibody; PRKM1 antibody; PRKM2 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_002745). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110362(ERK1/2 antibody) at dilution of 1:3000
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:110362(ERK1/2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:110362(ERK1/2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:110362(ERK1/2 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
-
Background
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to insulin and NGF. This antibody can recognize both ERK1 and ERK2.
-
References
- Zhang Y, Wang S, Li D. A systems biology-based classifier for hepatocellular carcinoma diagnosis. PloS one. 6(7):e22426. 2011.
- Bao YN, Cao X, Luo DH. Urokinase-type plasminogen activator receptor signaling is critical in nasopharyngeal carcinoma cell growth and metastasis. Cell cycle (Georgetown, Tex.). 13(12):1958-69. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"