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Product Name
ERI1 antibody
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Description
ERI1 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human liver cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in K-562 cells, HepG2 cells, HL-60 cells. Observed molecular weight by Western-blot: 46kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
3 5 exonuclease ERI1 antibody; 3 5 exoribonuclease 1 antibody; 3EXO antibody; 3'HEXO antibody; Eri 1 homolog antibody; ERI1 antibody; exoribonuclease 1 antibody; HEXO antibody; Histone mRNA 3 exonuclease 1 antibody; Protein 3hExo antibody; THEX1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ERI1 recombinant protein (Accession Number: NM_153332). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:110360(ERI1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human testis using Catalog No:110360(ERI1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:110360(ERI1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ERI1, also named as HEXO, 3'HEXO and THEX1, contains one exonuclease domain and one SAP domain. The SAP domain is necessary for binding to the stem-loop structure of histone mRNAs and to form the ternary complex with SLBP, but not for 3'-end histone mRNA exonuclease activity. It is a RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. It is able to bind other mRNAs. ERI1 is required for 5.8S rRNA 3'-end processing. It also binds to 5.8s ribosomal RNA. ERI1 binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.
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