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Product Name
ERCC8 Polyclonal Antibody
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Description
Polyclonal antibody to ERCC8
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
ERCC8 antibody; CKN1 antibody; CSA antibody; UVSS2 antibody; DNA excision repair protein ERCC-8 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human ERCC8
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100 -
Validations
Western blot - ERCC8 Polyclonal Antibody
Western blot analysis of extracts of mouse spleen, using ERCC8 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
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Background
Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"