-
Product Name
ERCC4 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to ERCC4
-
Tested applications
WB, IP
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
ERCC4 antibody; ERCC11 antibody; FANCQ antibody; RAD1 antibody; XFEPS antibody; XPF antibody; DNA repair endonuclease XPF antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 600-700 of human ERCC4 (NP_005227.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IP 1:20 - 1:50 -
Validations
Western blot - ERCC4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ERCC4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunoprecipitation - ERCC4 Polyclonal Antibody
Immunoprecipitation analysis of 150ug extracts of 293T cells using 3ug ERCC4 antibody . Western blot was performed from the immunoprecipitate using ERCC4 antibody at a dilition of 1:1000.
-
Background
Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"