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ERCC3 antibody

Cat.#: 110409

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Special Price 239.0 USD

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Product Information

  • Product Name
    ERCC3 antibody
  • Documents
  • Description
    ERCC3 Rabbit Polyclonal antibody. Positive IP detected in A431 cells. Positive WB detected in COLO 320 cells, A431 cells, HEK-293 cells, HeLa cells, HT-1080 cells, mouse colon tissue. Observed molecular weight by Western-blot: 89 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    BTF2 antibody; BTF2 p89 antibody; ERCC3 antibody; GTF2H antibody; RAD25 antibody; TFIIH antibody; TFIIH 89 kDa subunit antibody; TFIIH p89 antibody; XPB antibody; XPBC antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ERCC3 recombinant protein (Accession Number: NM_000122). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

  • Validations

    COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:110409(ERCC3 antibody) at dilution of 1:200

    COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:110409(ERCC3 antibody) at dilution of 1:200

    IP Result of anti-ERCC3 (IP:Catalog No:110409, 4ug; Detection:Catalog No:110409 1:500) with A431 cells lysate 1200ug.

    IP Result of anti-ERCC3 (IP:Catalog No:110409, 4ug; Detection:Catalog No:110409 1:500) with A431 cells lysate 1200ug.

  • Background
    Excision repair cross-complementing 3/xeroderma pigmentosum B (ERCC3/XPB) DNA helicase, is an essential ATP-dependent DNA helicase that functions as an integral component of the transcription factor IIH complex. ERCC3 is involved in initiation of basal transcription and global genome repair as well as in transcription-coupled repair (TCR) [PMID:19942596]. At the cellular level, a defect in ERCC3 results in high UV sensitivity, reduced post-UV RNA synthesis recovery and decreased DNA repair due to defective NER [PMID:16947863].
  • References
    • Xie QH, He XX, Chang Y. MiR-192 inhibits nucleotide excision repair by targeting ERCC3 and ERCC4 in HepG2.2.15 cells. Biochemical and biophysical research communications. 410(3):440-5. 2011.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"