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Product Name
EPPIN Polyclonal Antibody
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Description
Polyclonal antibody to EPPIN
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
EPPIN antibody; CT71 antibody; CT72 antibody; SPINLW1 antibody; WAP7 antibody; WFDC7 antibody; dJ461P17.2 antibody; eppin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-133 of human EPPIN (NP_065131.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - EPPIN Polyclonal Antibody
Western blot analysis of extracts of mouse testis, using EPPIN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .
Immunofluorescence - EPPIN Polyclonal Antibody
Immunofluorescence analysis of HepG2 cells using EPPIN antibody . Blue: DAPI for nuclear staining.
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Background
Serine protease inhibitor that plays an essential role in male reproduction and fertility. Modulates the hydrolysis of SEMG1 by KLK3/PSA (a serine protease), provides antimicrobial protection for spermatozoa in the ejaculate coagulum, and binds SEMG1 thereby inhibiting sperm motility.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"