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Product Name
EPHX2 antibody
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Description
EPHX2 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HeLa cells, HEK-293 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 63 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
CEH antibody; Cytosolic epoxide hydrolase antibody; EPHX2 antibody; Epoxide hydratase antibody; Epoxide hydrolase 2 antibody; SEH antibody; Soluble epoxide hydrolase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EPHX2 recombinant protein (Accession Number: NM_001979). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110387(EPHX2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:110387(EPHX2 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-EPHX2 (IP:Catalog No:110387, 4ug; Detection:Catalog No:110387 1:500) with HEK-293 cells lysate 2800ug.
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Background
EPHX2(Epoxide hydrolase 2) acts on epoxides (alkene oxides, oxiranes) and arene oxides and plays a role in xenobiotic metabolism by degrading potentially toxic epoxides.It was observed in mant tissues with the band of 63 Kda in the western blot.It has also been reported that the N-terminal domain might promote dimerization of EPHX2(PMID:21553642).
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References
- Labitzke EM, Diani-Moore S, Rifkind AB. Mitochondrial P450-dependent arachidonic acid metabolism by TCDD-induced hepatic CYP1A5; conversion of EETs to DHETs by mitochondrial soluble epoxide hydrolase. Archives of biochemistry and biophysics. 468(1):70-81. 2007.
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