Human EphA1/Eph Receptor A1 (His Tag) recombinant protein

Human EphA1/Eph Receptor A1 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.

Ephrin type-A receptor 1

MGC163163; 5730453L17RIK; AL033318; ESK; EPHT; EPHT1; EPH

P21709

EPHA1; EPH; EPHT; EPHT1

Human Cells

A DNA sequence encoding the human EPHA1 (EAL23789.1) (Met1-Glu547) was expressed with a polyhistidine tag at the C-terminus.

Human

The recombinant human EPHA1 consists 533 amino acids and predicts a molecular mass of 58 kDa.

> 95 % as determined by SDS-PAGE.

Measured by its binding ability in a functional ELISA.
Immobilized human EPHA1-His at 10 μg/ml (100 μl /well) can bind human EFNA1-Fch (Cat:503293 ), The EC₅₀ of human EFNA1-Fch (Cat:503293 ) is 30-70 ng/ml.