Mouse EphA1/Eph Receptor A1 (His Tag) recombinant protein

Mouse EphA1/Eph Receptor A1 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.

Ephrin type-A receptor 1

5730453L17RIK; EPHT; ESK; EPHT1; EPH; AL033318; MGC163163

Q60750

Epha1; Esk

Human Cells

A DNA sequence encoding the extracellular domain of mouse EPHA1 (Q60750) (Met 1-Glu 548) was expressed, with a C-terminal polyhistidine tag.

Mouse

The secreted recombinant mouse EPHA1 comprises 535 amino acids and has a calculated molecular mass of 58.6 kDa. As a result of glycosylation, the apparent molecular mass of rmEPHA1 is approximately 68 kDa in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized mouse EPHA1-His at 10 μg/ml (100 μl/well) can bind mouse EFNA1-Fc (Cat:503668), The EC₅₀ of mouse EFNA1-Fc (Cat:503668) is 21.3-49.8 ng/ml.