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Product Name
EPHA4 antibody
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Description
EPHA4 Rabbit Polyclonal antibody. Positive IF detected in Neuron cells. Positive FC detected in SH-SY5Y cells. Positive WB detected in mouse brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 120 kDa
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Tested applications
ELISA, WB, IF, IP, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
EK8 antibody; EPH like kinase 8 antibody; EPH receptor A4 antibody; EPHA4 antibody; Ephrin type A receptor 4 antibody; HEK8 antibody; SEK antibody; TYRO1 antibody; Tyrosine protein kinase TYRO1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EPHA4 recombinant protein (Accession Number: XM_005246374). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:50-1:500
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Validations
IF result of EPHA4 antibody (Catalog No:110349, 1:50) with culture neuron cells by Wilson Pak Kin Lou.
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110349(EPHA4 antibody) at dilution of 1:1000
IP Result of anti-EPHA4 (IP:Catalog No:110349, 4ug; Detection:Catalog No:110349 1:1000) with mouse brain tissue lysate 4000ug.
1X10^6 SH-SY5Y cells were stained with 0.2ug EPHA4 antibody (Catalog No:110349, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
EPHA4 represents EPH receptor A4
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"