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EPCAM antibody

Cat.#: 107254

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Product Information

  • Product Name
    EPCAM antibody
  • Documents
  • Description
    EPCAM Mouse Monoclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human breast cancer tissue. Positive WB detected in COLO 320 cells. Observed molecular weight by Western-blot: 35-40 kDa
  • Tested applications
    ELISA, IHC, WB, IF
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    CD326 antibody; CO 17A antibody; CO17 1A antibody; EGP antibody; EGP 2 antibody; EGP314 antibody; EGP34 antibody; EGP40 antibody; Ep CAM antibody; EPCAM antibody; Epithelial glycoprotein antibody; Epithelial glycoprotein 314 antibody; ESA antibody; GA733 2 antibody; hEGP 2 antibody; hEGP314 antibody; KS 1/4 antigen antibody; KS1/4 antibody; KSA antibody; M1S2 antibody; M4S1 antibody; MIC18 antibody; MK 1 antibody; TACST 1 antibody; TACSTD1 antibody; TROP1 antibody
  • Isotype
    Mouse IgG2a
  • Preparation
    This antibody was obtained by immunization of EPCAM recombinant protein (Accession Number: NM_002354). Purification method: Protein A purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IHC: 1:50-1:500

    IF: 1:50-1:500

  • Validations

    COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:107254(EPCAM Antibody) at dilution of 1:2000

    COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:107254(EPCAM Antibody) at dilution of 1:2000

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (EPCAM Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (EPCAM Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (EPCAM Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (EPCAM Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunofluorescent analysis of (-20oc Ethanol) fixed MCF-7 cells using Catalog No:107254(EPCAM Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

    Immunofluorescent analysis of (-20oc Ethanol) fixed MCF-7 cells using Catalog No:107254(EPCAM Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

  • Background
    Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. (PMID: 20837599; 19249674; 21576002; 22647938)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"