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Product Name
ENTPD1 antibody
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Description
ENTPD1 Rabbit Polyclonal antibody. Positive WB detected in mouse placenta tissue. Positive IHC detected in human placenta tissue, human liver tissue, human tonsil tissue, human tonsillitis tissue. Positive IF detected in HeLa cells. Positive FC detected in Jurkat cells. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, IHC, WB, FC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ATPDase antibody; CD39 antibody; DKFZp686D194 antibody; DKFZp686I093 antibody; Ecto apyrase antibody; Ecto ATP diphosphohydrolase 1 antibody; Ecto ATPase 1 antibody; Ecto ATPDase 1 antibody; ENTPD1 antibody; FLJ40921 antibody; FLJ40959 antibody; NTPDase 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ENTPD1 recombinant protein (Accession Number: NM_001164183). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human placenta using Catalog No:110330(ENTPD1 antibody) at dilution of 1:50 (under 40x lens)
mouse placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:110330(ENTPD1 Antibody) at dilution of 1:300
Immunofluorescent analysis of HeLa cells using Catalog No:110330(ENTPD1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 Jurkat cells were stained with 0.5ug ENTPD1 antibody (Catalog No:110330, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named as CD39, NTPDase 1, Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.It is a noncovalent tetrameric protein and that detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ).And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa and 34 kDa) produced by alternative splicing.
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