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Product Name
ENOX2 Polyclonal Antibody
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Description
Polyclonal antibody to ENOX2
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ENOX2 antibody; APK1 antibody; COVA1 antibody; tNOX antibody; ecto-NOX disulfide-thiol exchanger 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 411-610 of human ENOX2 (NP_872114.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - ENOX2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ENOX2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.
Immunofluorescence - ENOX2 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using ENOX2 antibody . Blue: DAPI for nuclear staining.
Immunofluorescence - ENOX2 Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using ENOX2 antibody . Blue: DAPI for nuclear staining.
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Background
May be involved in cell growth. Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"