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Product Name
ENO3 antibody
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Description
ENO3 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HeLa cells, HepG2 cells, mouse brain tissue, multi-cells/tissue, SH-SY5Y cells. Positive IHC detected in human skeletal muscle tissue, human heart tissue. Observed molecular weight by Western-blot: 42-47kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Beta enolase antibody; ENO3 antibody; Enolase 3 antibody; enolase 3 (beta antibody; muscle) antibody; MSE antibody; Muscle specific enolase antibody; Skeletal muscle enolase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001976). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
WB result of Catalog No:110254
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:110254(ENO3 antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:110254(ENO3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skeletal muscle using Catalog No:110254(ENO3 antibody) at dilution of 1:50 (under 40x lens)
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Background
ENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). This antibody is specific to ENO3. It has no cross reaction to ENO1 and ENO2.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"