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Product Name
ENO1 antibody
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Description
ENO1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human brain tissue, human pancreas tissue, human skeletal muscle tissue. Positive IP detected in mouse brain tissue. Positive WB detected in HeLa cells, HepG2 cells, mouse brain tissue, mouse liver tissue. Observed molecular weight by Western-blot: 47 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Alpha enolase antibody; C myc promoter binding protein antibody; ENO1 antibody; ENO1L1 antibody; Enolase 1 antibody; enolase 1 antibody; (alpha) antibody; MBP 1 antibody; MBPB1 antibody; MPB 1 antibody; MPB1 antibody; NNE antibody; Non neural enolase antibody; Phosphopyruvate hydratase antibody; Plasminogen binding protein antibody; PPH antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001428). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110252(ENO1 antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human brain using Catalog No:110252(ENO1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:110252(ENO1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ENO1 (IP:Catalog No:110252, 4ug; Detection:Catalog No:110252 1:500) with mouse brain tissue lysate 4000ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:110252(ENO1 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
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Background
ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. It is a multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. ENO1 may also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. It stimulates immunoglobulin production. (PMID:2005901). This antibody is specific to ENO1. It has no cross reaction to ENO2 and ENO3.
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