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Product Name
ENDOG Polyclonal Antibody
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Description
Polyclonal antibody to ENDOG
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
ENDOG antibody; endonuclease G antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 49-297 of human ENDOG (NP_004426.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:500
IHC 1:50 - 1:100 -
Validations
Western blot - ENDOG Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ENDOG antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - ENDOG Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using ENDOG antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ENDOG Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse lung using ENDOG antibody at dilution of 1:100 (40x lens).
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Background
Cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"