EMAP II antibody

Cat.#: 110234

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Product Information

  • Product Name
    EMAP II antibody
  • Documents
  • Description
    EMAP II Rabbit Polyclonal antibody. Positive IHC detected in human cervical cancer tissue. Positive IF detected in Hela cells. Positive WB detected in SGC-7901 cells, mouse pancreas tissue. Observed molecular weight by Western-blot: 34kd and 68kd
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    AIMP1 antibody; EMAP 2 antibody; EMAP II antibody; EMAP2 antibody; EMAPII antibody; p43 antibody; SCYE1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of EMAP II recombinant protein (Accession Number: XM_017008836). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with Catalog No:110234(SCYE1 antibody) at dilution of 1:500

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with Catalog No:110234(SCYE1 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:110234(SCYE1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:110234(SCYE1 antibody) at dilution of 1:100 (under 10x lens)

    Immunofluorescent analysis of Hela cells, using SCYE1 antibody Catalog No:110234 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of Hela cells, using SCYE1 antibody Catalog No:110234 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Background
    SCYE1(small inducible cytokine subfamily E, member 1) is also named as EMAP2, AIMP1. The human cDNA encodes a 312-amino acid polypeptide that the predicted 34-kD precursor can be cleaved to produce an active 20-kD product(PMID:7929199). SCYE1 is secreted as a result of various stimuli, including TNF-α, heat shock, and hypoxia, and that it acts as a multifunctional cytokine on both endothelial and immune cells(PMID:18292511). Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). It has 2 isoforms produced by alternative splicing and the protein can exsit as a dimer(PMID:11306575).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"