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Product Name
EMAP II antibody
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Description
EMAP II Rabbit Polyclonal antibody. Positive IHC detected in human cervical cancer tissue. Positive IF detected in Hela cells. Positive WB detected in SGC-7901 cells, mouse pancreas tissue. Observed molecular weight by Western-blot: 34kd and 68kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AIMP1 antibody; EMAP 2 antibody; EMAP II antibody; EMAP2 antibody; EMAPII antibody; p43 antibody; SCYE1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EMAP II recombinant protein (Accession Number: XM_017008836). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
SGC-7901 cells were subjected to SDS PAGE followed by western blot with Catalog No:110234(SCYE1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:110234(SCYE1 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of Hela cells, using SCYE1 antibody Catalog No:110234 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
SCYE1(small inducible cytokine subfamily E, member 1) is also named as EMAP2, AIMP1. The human cDNA encodes a 312-amino acid polypeptide that the predicted 34-kD precursor can be cleaved to produce an active 20-kD product(PMID:7929199). SCYE1 is secreted as a result of various stimuli, including TNF-α, heat shock, and hypoxia, and that it acts as a multifunctional cytokine on both endothelial and immune cells(PMID:18292511). Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). It has 2 isoforms produced by alternative splicing and the protein can exsit as a dimer(PMID:11306575).
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