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Product Name
ELMO3 Polyclonal Antibody
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Description
Polyclonal antibody to ELMO3
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
ELMO3 antibody; CED-12 antibody; CED12 antibody; ELMO-3 antibody; engulfment and cell motility 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 504-773 of human ELMO3 (NP_078988.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - ELMO3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ELMO3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
Immunohistochemistry - ELMO3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney using ELMO3 antibody at dilution of 1:100 (40x lens).
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Background
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1 (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"