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Product Name
ELL Polyclonal Antibody
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Description
Polyclonal antibody to ELL
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
ELL antibody; C19orf17 antibody; ELL1 antibody; MEN antibody; PPP1R68 antibody; RNA polymerase II elongation factor ELL antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human ELL
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:500
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Validations
Western blot - ELL Polyclonal Antibody
Western blot analysis of extracts of 293 cells, using ELL antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
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Background
Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Elongation factor component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III. Specifically required for stimulating the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription. ELL also plays an early role before its assembly into in the SEC complex by stabilizing RNA polymerase II recruitment/initiation and entry into the pause site. Required to stabilize the pre-initiation complex and early elongation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"