-
Product Name
ELK1 antibody
- Documents
-
Description
ELK1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in Jurkat cells, HepG2 cells, mouse pancreas tissue. Observed molecular weight by Western-blot: 62 kDa
-
Tested applications
ELISA, WB, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
ELK1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of ELK1 recombinant protein (Accession Number: XM_017029339). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:50-1:500
-
Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:110278(ELK1 antibody) at dilution of 1:800
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:110278(ELK1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
E twenty-six (ETS)-like transcription factor 1, also known as ELK1, is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. It is targeted in the nucleus by three major MAP kinase pathways as well as the CaMKII pathway. phosphorylation of nuclear Elk1 enhances its ability to bind DNA at serum response elements and stimulate transcription of immediate early genes. Elk1 plays important roles in various contexts, including long-term memory formation, drug addiction, Alzheimer’s disease, down syndrome, breast cancer, and depression. This antibody got an 62kd band in western blotting maybe duo to its phosphorylation.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"