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Product Name
ELF5 Polyclonal Antibody
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Description
Polyclonal antibody to ELF5
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ELF5 antibody; ESE2 antibody; E74 like ETS transcription factor 5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ELF5 (NP_001413.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - ELF5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ELF5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - ELF5 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using ELF5 antibody . Blue: DAPI for nuclear staining.
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Background
Transcriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation.; Isoform 2 binds to DNA sequences containing the consensus nucleotide core sequence GGA[AT]. Transcriptionally activates SPRR2A and the parotid gland-specific PSP promoters.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"