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Product Name
ELF3 Polyclonal Antibody
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Description
Polyclonal antibody to ELF3
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Tested applications
WB, IHC, IF, IP
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Species reactivity
Human, Mouse
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Alternative names
ELF3 antibody; EPR-1 antibody; ERT antibody; ESE-1 antibody; ESX antibody; E74 like ETS transcription factor 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ELF3 (NP_004424.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50 -
Validations
Western blot - ELF3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ELF3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - ELF3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse heart using ELF3 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - ELF3 Polyclonal Antibody
Immunofluorescence analysis of MCF7 cells using ELF3 antibody . Blue: DAPI for nuclear staining.
Immunoprecipitation - ELF3 Polyclonal Antibody
Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug ELF3 antibody . Western blot was performed from the immunoprecipitate using ELF3 antibody at a dilition of 1:500.____Detection: ECL Enhanced Kit ._Exposure time: 30s.
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Background
Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"