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Product Name
ELF2 Polyclonal Antibody
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Description
Polyclonal antibody to ELF2
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
ELF2 antibody; EU32 antibody; NERF antibody; NERF-1A antibody; NERF-1B antibody; NERF-1a antibody; b antibody; NERF-2 antibody; E74 like ETS transcription factor 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human ELF2 (NP_006865.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - ELF2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ELF2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
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Background
Isoform 1 transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter.; Isoform 2 may function in repression of RUNX1-mediated transactivation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"