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Product Name
ELANE Polyclonal Antibody
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Description
Polyclonal antibody to ELANE
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
ELANE antibody; ELA2 antibody; GE antibody; HLE antibody; HNE antibody; NE antibody; PMN-E antibody; SCN1 antibody; neutrophil elastase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-267 of human ELANE (NP_001963.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - ELANE Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ELANE antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
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Background
Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"