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Product Name
EIF5B Polyclonal Antibody
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Description
Polyclonal antibody to EIF5B
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Tested applications
IF
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Species reactivity
Human
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Alternative names
EIF5B antibody; IF2 antibody; eukaryotic translation initiation factor 5B antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 901-1220 of human EIF5B (NP_056988.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
IF 1:10 - 1:100
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Validations
Immunofluorescence - EIF5B Polyclonal Antibody
Immunofluorescence analysis of A549 cells using EIF5B antibody . Blue: DAPI for nuclear staining.
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Background
Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"