-
Product Name
EIF5A antibody
- Documents
-
Description
EIF5A Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 18 kDa
-
Tested applications
ELISA, WB, IF
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
eIF 4D antibody; EIF 5A antibody; eIF 5A 1 antibody; eIF 5A1 antibody; EIF5A antibody; EIF5A1 antibody; eIF5AI antibody; Rev binding factor antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of EIF5A recombinant protein (Accession Number: NM_001370420). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:20-1:200
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110267(EIF5A antibody) at dilution of 1:400
Immunofluorescent analysis of HeLa cells using Catalog No:110267(EIF5A Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
Translation of messenger RNA (mRNA) to protein in eukaryotesis a crucial process in protein biosynthesis, in which initiation of translation involves interaction of different eukaryotic translation initiation factors (eIFs), ribosome subunits and mRNAs. Eukaryotic translation initiation factor 5A (EIF5A) is one of the eIFs involved in translation initiation. EIF5A has a essential role in cell viability and the only protein known to contain the amino acid residue hypusine, formed by post-translational modification of a specific lysine residue. Firstly identified as a translation initiation factor, it also has a function in the elongation step of translation.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"