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Product Name
EIF4H Polyclonal Antibody
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Description
Polyclonal antibody to EIF4H
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
EIF4H antibody; WBSCR1 antibody; WSCR1 antibody; eIF-4H antibody; eukaryotic translation initiation factor 4H antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human EIF4H (NP_114381.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - EIF4H Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using EIF4H antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Stimulates the RNA helicase activity of EIF4A in the translation initiation complex. Binds weakly mRNA.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"