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Product Name
EIF4G3 antibody
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Description
EIF4G3 Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive WB detected in HEK-293 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 70,250 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
eIF 4 gamma 3 antibody; eIF 4 gamma II antibody; eIF 4G 3 antibody; eIF4G 3 antibody; EIF4G3 antibody; eIF4GII antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EIF4G3 recombinant protein (Accession Number: NM_001198803). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:110264(EIF4G3 antibody) at dilution of 1:10 (under 10x lens)
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:110264(EIF4G3 Antibody) at dilution of 1:600
IP Result of anti-EIF4G3 (IP:Catalog No:110264, 4ug; Detection:Catalog No:110264 1:500) with HeLa cells lysate 4000ug.
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Background
Eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3) may be involved in the recognition of the mRNA cap. The protein migrated at approximately 250 kDa in SDS-PAGE.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"