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Product Name
EIF4A3 antibody
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Description
EIF4A3 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human brain tissue, human heart tissue, human lung tissue, human ovary tissue, human spleen tissue, human testis tissue. Positive IF detected in MCF-7 cells. Positive IP detected in mouse heart tissue. Positive WB detected in A549 cells, HEK-293 cells, HeLa cells, human placenta tissue, MCF7 cells, mouse heart tissue, mouse liver tissue, mouse lung tissue, mouse thymus tissue. Observed molecular weight by Western-blot: 47 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DDX48 antibody; DEAD box protein 48 antibody; eIF 4A III antibody; eIF4A III antibody; EIF4A3 antibody; eIF4AIII antibody; hNMP 265 antibody; hNMP265 antibody; KIAA0111 antibody; Nuclear matrix protein 265 antibody; NUK 34 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EIF4A3 recombinant protein (Accession Number: NM_014740). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:110257(EIF4A3 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human kidney using Catalog No:110257(EIF4A3 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using EIF4A3 antibody Catalog No:110257 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-EIF4A3 (IP:Catalog No:110257, 3ug; Detection:Catalog No:110257 1:1000) with mouse heart tissue lysate 5000ug.
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Background
EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions
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References
- Meikar O, Vagin VV, Chalmel F. An atlas of chromatoid body components. RNA (New York, N.Y.). 20(4):483-95. 2014.
- Melero R, Uchiyama A, Castaño R. Structures of SMG1-UPFs complexes: SMG1 contributes to regulate UPF2-dependent activation of UPF1 in NMD. Structure (London, England : 1993). 22(8):1105-19. 2014.
- Fletcher BR, Hill GS, Long JM, Gallagher M, Shapiro ML, Rapp PR. A fine balance: Regulation of hippocampal Arc/Arg3.1 transcription, translation and degradation in a rat model of normal cognitive aging. Neurobiology of learning and memory. 115:58-67. 2014.
- Jang MK, Anderson DE, van Doorslaer K, McBride AA. A proteomic approach to discover and compare interacting partners of papillomavirus E2 proteins from diverse phylogenetic groups. Proteomics. 15(12):2038-50. 2015.
- Chuang TW, Lee KM, Lou YC, Lu CC, Tarn WY. A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement. The Journal of biological chemistry. 2016.
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