EIF4A2 Polyclonal Antibody

Cat.#: 164220

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Product Information

  • Product Name
    EIF4A2 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to EIF4A2
  • Tested applications
    WB, IHC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    EIF4A2 antibody; BM-010 antibody; DDX2B antibody; EIF4A antibody; EIF4F antibody; eIF-4A-II antibody; eIF4A-II antibody; eukaryotic initiation factor 4A-II antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EIF4A2 (NP_001958.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:1000
    IHC 1:50 - 1:200
  • Validations

    Western blot - EIF4A2 Polyclonal Antibody

    Western blot - EIF4A2 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using EIF4A2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.

    Immunohistochemistry - EIF4A2 Polyclonal Antibody

    Immunohistochemistry - EIF4A2 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded rat brain using EIF4A2 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - EIF4A2 Polyclonal Antibody

    Immunohistochemistry - EIF4A2 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded rat heart using EIF4A2 antibody at dilution of 1:100 (40x lens).

    Immunohistochemistry - EIF4A2 Polyclonal Antibody

    Immunohistochemistry - EIF4A2 Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded mouse brain using EIF4A2 antibody at dilution of 1:100 (40x lens).

  • Background
    ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"