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Product Name
EIF4A2 Polyclonal Antibody
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Description
Polyclonal antibody to EIF4A2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
EIF4A2 antibody; BM-010 antibody; DDX2B antibody; EIF4A antibody; EIF4F antibody; eIF-4A-II antibody; eIF4A-II antibody; eukaryotic initiation factor 4A-II antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human EIF4A2 (NP_001958.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:200 -
Validations
Western blot - EIF4A2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using EIF4A2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - EIF4A2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using EIF4A2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - EIF4A2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using EIF4A2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - EIF4A2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using EIF4A2 antibody at dilution of 1:100 (40x lens).
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Background
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"