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Product Name
EIF3J Polyclonal Antibody
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Description
Polyclonal antibody to EIF3J
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
EIF3J antibody; EIF3S1 antibody; eIF3-alpha antibody; eIF3-p35 antibody; eukaryotic translation initiation factor 3 subunit J antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human EIF3J (NP_003749.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - EIF3J Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using EIF3J antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunohistochemistry - EIF3J Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using EIF3J antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - EIF3J Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung using EIF3J antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - EIF3J Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using EIF3J antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - EIF3J Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse spleen using EIF3J antibody at dilution of 1:100 (40x lens).
Immunofluorescence - EIF3J Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using EIF3J antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - EIF3J Polyclonal Antibody
Immunofluorescence analysis of NIH/3T3 cells using EIF3J antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - EIF3J Polyclonal Antibody
Immunofluorescence analysis of PC-12 cells using EIF3J antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"