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Product Name
EIF3I antibody
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Description
EIF3I Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells, HepG2 cells, human placenta tissue, Jurkat cells, K-562 cells, MCF7 cells, mouse brain tissue, NIH/3T3 cells, SH-SY5Y cells. Positive IP detected in HEK-293 cells. Positive FC detected in HeLa cells. Positive IF detected in Hela cells. Positive IHC detected in human breast cancer tissue, human liver tissue, human testis tissue. Observed molecular weight by Western-blot: 36kd
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Tested applications
ELISA, IHC, IF, IP, WB, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
eIF 3 beta antibody; eIF3 beta antibody; eIF3 p36 antibody; EIF3I antibody; EIF3S2 antibody; PRO2242 antibody; TRIP 1 antibody; TRIP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EIF3I recombinant protein (Accession Number: NM_003757). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:110197(EIF3I antibody) at dilution of 1:1000
IP Result of anti-EIF3I (IP:Catalog No:110197, 3ug; Detection:Catalog No:110197 1:1000) with HEK-293 cells lysate 1500ug.
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:110197(EIF3I antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of Hela cells, using EIF3I antibody Catalog No:110197 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HeLa cells were stained with 0.2ug EIF3I antibody (Catalog No:110197, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Eukaryotic initiation factor (EIF3) complex is the largest of the eIFs and consists of more than 10 nonidentical subunit, and is required for several steps in the initiation of protein synthesis. It associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). Also it can stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3I is one subunit of EIF3 complex.
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References
- Metz-Estrella D, Jonason JH, Sheu TJ, Mroczek-Johnston RM, Puzas JE. TRIP-1: a regulator of osteoblast function. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 27(7):1576-84. 2012.
- Roobol A, Roobol J, Carden MJ. The chaperonin CCT interacts with and mediates the correct folding and activity of three subunits of translation initiation factor eIF3: b, i and h. The Biochemical journal. 458(2):213-24. 2014.
- Mead EJ, Masterton RJ, Feary M. Biological insights into the expression of translation initiation factors from recombinant CHOK1SV cell lines and their relationship to enhanced productivity. The Biochemical journal. 472(3):261-73. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"