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Product Name
EIF3H antibody
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Description
EIF3H Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 40kd
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Tested applications
ELISA, WB
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
EIF3H antibody; eIF 3 gamma antibody; eIF3 p40 subunit antibody; eIF3 gamma antibody; EIF3S3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EIF3H recombinant protein (Accession Number: NM_003756). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
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Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:110196(EIF3H antibody) at dilution of 1:500
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Background
Eukaryotic translation initiation factor eIF3, that plays a central role in translation initiation, consists of five core subunits that are present in both the budding yeast and higher eukaryotes. The EIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex. The complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. It is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3H is a component of the EIF3 complex. [PMID:19204574,20503360]
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