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Product Name
EIF3D antibody
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Description
EIF3D Mouse Monoclonal antibody. Positive WB detected in HepG2 cells, A431 cells, HeLa cells, human brain tissue, mouse liver tissue, rat liver tissue. Positive IF detected in HeLa cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 66 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
eIF 3 zeta antibody; eIF3 p66 antibody; eIF3 zeta antibody; EIF3D antibody; EIF3S7 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of EIF3D recombinant protein (Accession Number: NM_003753). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107251(EIF3D antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:107251(EIF3D antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:107251(EIF3D antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:107251(EIF3D Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.
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