EIF3D antibody

Cat.#: 107251

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Product Information

  • Product Name
    EIF3D antibody
  • Documents
  • Description
    EIF3D Mouse Monoclonal antibody. Positive WB detected in HepG2 cells, A431 cells, HeLa cells, human brain tissue, mouse liver tissue, rat liver tissue. Positive IF detected in HeLa cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 66 kDa
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    eIF 3 zeta antibody; eIF3 p66 antibody; eIF3 zeta antibody; EIF3D antibody; EIF3S7 antibody
  • Isotype
    Mouse IgG2b
  • Preparation
    This antibody was obtained by immunization of EIF3D recombinant protein (Accession Number: NM_003753). Purification method: Protein A purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

    IF: 1:50-1:500

  • Validations

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107251(EIF3D antibody) at dilution of 1:1000

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107251(EIF3D antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human brain using Catalog No:107251(EIF3D antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human brain using Catalog No:107251(EIF3D antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human brain using Catalog No:107251(EIF3D antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human brain using Catalog No:107251(EIF3D antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:107251(EIF3D Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:107251(EIF3D Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

  • Background
    The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"