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Product Name
EIF1AY antibody
- Documents
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Description
EIF1AY Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, HEK-293 cells. Positive IHC detected in human prostate cancer tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 16-20 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
eIF 1A Y isoform antibody; eIF 4C antibody; EIF1AY antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EIF1AY recombinant protein (Accession Number: NM_004681). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:110176(EIF1AY antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:110176(EIF1AY antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells using Catalog No:110176(EIF1AY Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
EIF1AY is 1 of 5 NRY genes that are expressed in many tissues and have homologs on the X chromosome, which is an essential translation initiation factor. EIF1AY seems to be required for maximal rate of protein biosynthesis, and enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits. This is a rabbit polyclonal antibody raised against the full length of human EIF1AY, and can recognize both EIF1AY and EIF1AX.
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