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Product Name
EID1 antibody
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Description
EID1 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, A431 cells, HT-1080 cells, human skeletal muscle tissue, K-562 cells, U-937 cells. Positive IHC detected in human breast cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 20-22kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
21 kDa pRb assOCiated protein antibody; C15orf3 antibody; CRI1 antibody; EID 1 antibody; EID1 antibody; IRO45620 antibody; PNAS 22 antibody; PTD014 antibody; RBP21 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EID1 recombinant protein (Accession Number: BC024151). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:110170(EID1 antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:110170(EID1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:110170(EID1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:110170(EID1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
EID1 represents EP300 interacting inhibitor of differentiation 1
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References
- Liao H, Liu XJ, Blank JL. Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924. Molecular & cellular proteomics : MCP. 10(11):M111.009183. 2011.
- Yoshida Y, Saeki Y, Murakami A. A comprehensive method for detecting ubiquitinated substrates using TR-TUBE. Proceedings of the National Academy of Sciences of the United States of America. 112(15):4630-5. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"