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Product Name
EGR2 antibody
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Description
EGR2 Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, human kidney tissue, mouse cerebellum tissue. Observed molecular weight by Western-blot: 50kd
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Tested applications
ELISA, WB
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Species reactivity
human, mouse, rat; other species not tested.
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Alternative names
EGR2 antibody; KROX20 antibody; Zinc finger protein Krox 20 antibody; EGR 2 antibody; DKFZp686J1957 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EGR2 recombinant protein (Accession Number: BC035625). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110221(EGR2 antibody) at dilution of 1:1000
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Background
Egr2 belongs to the Egr family that binds to DNA through highly conserved zinc finger domains and has a pivotal role in hindbrain development and myelination of the peripheral nervous system, development of T and/or B cells[PMID:19169262] . It has been demonstrated that Egr2 is induced early upon TCR engagement in a NFAT-dependent manner, and that overexpression of Egr2 inhibits T cell activation[PMID:23129747]. Overexpressing Egr2 shows an upregulation of E3-ligase Cbl-band reduced production of interleukin-2 (IL-2), suggesting that Egr2 is important for the maintenance of T cell tolerance by negatively regulating T cell activation[PMID: 15834410].
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References
- Wang SS, Huang HY, Chen SZ. Early growth response 2 (Egr2) plays opposing roles in committing C3H10T1/2 stem cells to adipocytes and smooth muscle-like cells. The international journal of biochemistry & cell biology. 45(8):1825-32. 2013.
- Ogawa M, Okamura T, Ishikura S. Zfat-deficiency results in a loss of CD3ζ phosphorylation with dysregulation of ERK and Egr activities leading to impaired positive selection. PloS one. 8(10):e76254. 2013.
- Gökbuget D, Pereira JA, Bachofner S. The Lin28/let-7 axis is critical for myelination in the peripheral nervous system. Nature communications. 6:8584. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"