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Product Name
EGFR-Specific antibody
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Description
EGFR-Specific Rabbit Polyclonal antibody. Positive IP detected in MCF-7 cells. Positive WB detected in HeLa cells, A431 cells, A549 cells, HeLa/RPE1 cells, MCF7 cells. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 145-165 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human, Rat; other species not tested.
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Alternative names
EGFR antibody; ERBB antibody; ERBB1 antibody; HER1 antibody; mENA antibody; PIG61 antibody; Proto oncogene c ErbB 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_005228). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
WB result of anti-EGFR (Catalog No:110218) in different cell lysates.
WB result ofanti-EGFR in Hela and REP1 cell by Dr.Kodani, Andrew.
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:110218(EGFR-Specific antibody) at dilution of 1:1000
IP Result of anti-EGFR-Specific (IP:Catalog No:110218, 3ug; Detection:Catalog No:110218 1:500) with MCF-7 cells lysate 2500ug.
Immunofluorescent analysis of Hela cells, using EGFR antibody Catalog No:110218 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
ERBB1, also mamed as EGFR, is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer. The antibody is specific to isoform1.
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References
- Zhang QC, Jiang SJ, Zhang S, Ma XB. Histone deacetylase inhibitor trichostatin A enhances anti-tumor effects of docetaxel or erlotinib in A549 cell line. Asian Pacific journal of cancer prevention : APJCP. 13(7):3471-6. 2012.
- Zhang Z, Miao L, Sun W. Wentilactone B from Aspergillus wentii induces apoptosis and inhibits proliferation and migration of human hepatoma SMMC-7721 cells. Biological & pharmaceutical bulletin. 35(11):1964-71. 2012.
- Ning Y, Shang Y, Huang H. Attenuation of cigarette smoke-induced airway mucus production by hydrogen-rich saline in rats. PloS one. 8(12):e83429. 2013.
- Li X, Wan X, Chen H. Identification of miR-133b and RB1CC1 as independent predictors for biochemical recurrence and potential therapeutic targets for prostate cancer. Clinical cancer research : an official journal of the American Association for Cancer Research. 20(9):2312-25. 2014.
- Xie J, Chen M, Zhou J. miR-7 inhibits the invasion and metastasis of gastric cancer cells by suppressing epidermal growth factor receptor expression. Oncology reports. 31(4):1715-22. 2014.
- Dai B, Zhan Y, Qi J, Zhang Y. Eupolyphaga sinensis Walker inhibits human chronic myeloid leukemia cell K562 growth by inducing G2-M phase cell cycle arrest and targeting EGFR signaling pathway and in S180 tumor-bearing mice. Environmental toxicology and pharmacology. 37(3):1177-85. 2014.
- Zhang MZ, Li C, Fang BY. High transfection efficiency of quantum dot-antisense oligonucleotide nanoparticles in cancer cells through dual-receptor synergistic targeting. Nanotechnology. 25(25):255102. 2014.
- Su Z, Yin J, Zhao L. Lentiviral vector-mediated RBM5 overexpression downregulates EGFR expression in human non-small cell lung cancer cells. World journal of surgical oncology. 12:367. 2014.
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