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Product Name
EFHD2 Polyclonal Antibody
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Description
Polyclonal antibody to EFHD2
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse
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Alternative names
EFHD2 antibody; SWS1 antibody; EF-hand domain family member D2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-78 of human EFHD2 (NP_077305.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - EFHD2 Polyclonal Antibody
Western blot analysis of extracts of mouse lung, using EFHD2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - EFHD2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human tonsil using EFHD2 antibody at dilution of 1:150 (40x lens).
Immunohistochemistry - EFHD2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human appendix using EFHD2 antibody at dilution of 1:150 (40x lens).
Immunohistochemistry - EFHD2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse spleen using EFHD2 antibody at dilution of 1:150 (40x lens).
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Background
May regulate B-cell receptor (BCR)-induced immature and primary B-cell apoptosis. Plays a role as negative regulator of the canonical NF-kappa-B-activating branch. Controls spontaneous apoptosis through the regulation of BCL2L1 abundance.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"