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Product Name
EEF1D Polyclonal Antibody
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Description
Polyclonal antibody to EEF1D
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
EEF1D antibody; EF-1D antibody; EF1D antibody; FP1047 antibody; elongation factor 1-delta antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human EEF1D (NP_001123529.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - EEF1D Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using EEF1D antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - EEF1D Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using EEF1D antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - EEF1D Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using EEF1D antibody at dilution of 1:200 (40x lens).
Immunofluorescence - EEF1D Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using EEF1D antibody . Blue: DAPI for nuclear staining.
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Background
Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.; Isoform 2: Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"