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Product Name
EEF1D antibody
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Description
EEF1D Mouse Monoclonal antibody. Positive IF detected in MCF-7. Positive IHC detected in human pancreas cancer. Observed molecular weight by Western-blot: 31/71kd
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Tested applications
ELISA, IHC, IF
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Species reactivity
human, mouse; other species not tested.
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Alternative names
EEF1D antibody; EF 1 delta antibody; EF 1D antibody; FP1047 antibody; Elongation factor 1 delta antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of EEF1D recombinant protein (Accession Number: XM_047421433). Purification method: Caprylic acid/ammonium sulfate precipitation.
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Clonality
Monoclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:107249(EEF1D antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:107249(EEF1D antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using EEF1D antibody Catalog No: at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
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Background
EEF1D, also named as EF1D and EF 1 delta, belongs to the EF-1-beta/EF-1-delta family. It is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP. EEF1D is phosphorylated upon DNA damage, probably by ATM or ATR. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human EEF1D.
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