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Product Name
EEF1B2 antibody
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Description
EEF1B2 Rabbit Polyclonal antibody. Positive WB detected in SKOV-3 cells, HEK-293 cells, HeLa cells, Jurkat cells, PC-3 cells, RAW264.7 cells. Positive IP detected in Jurkat cells. Positive IF detected in MCF-7 cells. Positive IHC detected in human pancreas cancer tissue. Observed molecular weight by Western-blot: 24-30 kDa, 34 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
EEF1B antibody; EEF1B1 antibody; EEF1B2 antibody; EF 1 beta antibody; EF1B antibody; Elongation factor 1 beta antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of EEF1B2 recombinant protein (Accession Number: NM_021121). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
SKOV-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:110313(EEF1B2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:110313(EEF1B2 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using Catalog No:110313 and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-EEF1B2 (IP:Catalog No:110313, 4ug; Detection:Catalog No:110313 1:500) with Jurkat cells lysate 2400ug.
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Background
In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30 kDa protein in SDS-PAGE. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human EEF1B2.
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References
- Cao Y, Portela M, Janikiewicz J, Doig J, Abbott CM. Characterisation of translation elongation factor eEF1B subunit expression in mammalian cells and tissues and co-localisation with eEF1A2. PloS one. 9(12):e114117. 2014.
- Mazan-Mamczarz K, Lal A, Martindale JL, Kawai T, Gorospe M. Translational repression by RNA-binding protein TIAR. Molecular and cellular biology. 26(7):2716-27. 2006.
- Mazan-Mamczarz K, Kawai T, Martindale JL, Gorospe M. En masse analysis of nascent translation using microarrays. BioTechniques. 39(1):61-2, 64, 66-7. 2005.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"