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Product Name
EDN3 Polyclonal Antibody
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Description
Polyclonal antibody to EDN3
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Tested applications
WB
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Species reactivity
Human
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Alternative names
EDN3 antibody; ET-3 antibody; ET3 antibody; HSCR4 antibody; PPET3 antibody; WS4B antibody; endothelin-3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 17-187 of human EDN3 (NP_996917.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - EDN3 Polyclonal Antibody
Western blot analysis of extracts of A-549 cells, using EDN3 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 20s.
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Background
Endothelins are endothelium-derived vasoconstrictor peptides.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"