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Product Name
EDC4 Polyclonal Antibody
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Description
Polyclonal antibody to EDC4
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
EDC4 antibody; GE1 antibody; Ge-1 antibody; HEDL5 antibody; HEDLS antibody; RCD-8 antibody; RCD8 antibody; enhancer of mRNA decapping 4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 800-1100 of human EDC4 (NP_055144.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
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Validations
Western blot - EDC4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using EDC4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
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Background
In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"